Evaluation of the effect of a oligod manan saccharide on the zootechnical, microbiological, pathological and histological parameters in broilers related to salmonella enteritidis

Abstract

This study was undertaken to assess the effect of the combination of an oligod manan saccharide 1 g/kg diet, compared to a commercial antibiotic, Bacitracin Zinc, 2,2 mg/kg of feed used in the diet of broilers from 0 to 20 days. Broilers were divided into five experimental groups with three replicates per treatment as follows: treatment 1 (T1), positive control inoculated with Salmonella enteritidis (SE); treatment 2 (T2), treated with oligod manan saccharide; treatment 3 (T3), oligod manan saccharide treated with antibiotic therapy; treatment 4 (T4), treated with antibiotics; treatment 5 (T5), without treatment as negative control. The first four treatments were challenged with 4x109 CFU/ml of Salmonella enteritidis (SE), 5 and 14 days after birth. Productive parameters were recorded weekly. For microbiological parameters also were collected weekly stool samples in order to carry out the respective microbiological testing. As for the histological parameters, samples of thymus, liver, spleen, bone, small intestine and Fabricio bag to perform the histological analysis. The results obtained after performing 15 experiments and evaluate fifteen productive parameters indicated that the best treatment was 3, with a conversion of 1,278, the weight to 21 days on average of 762 grams and $ 54,76 pesos per kilogram of gain per animal. The second best treatment was 2. Regarding microbiological tests was an increase in lactic acid bacteria by the addition of MOS in treatments 2, 3 and 4, decreasing to 20 days. In treatments 2 and 3 the number of bacteria significantly increased from day 10, the number of bacteria in feces of Salmonella enteritidis, with no pathological evidence of disease present, there was also increase in Escherichia coli. With reference to the histological parameters, we note that the oligod manan saccharide protects the intestinal wall, which was evident in the treatment 2 and 3 (p≤0,05); it indicates that, histologically, treatments with the best answer were 2 and 3 (p≤0,05), with more cryptic growth than the other groups worked